Magzol reagent
WebAug 17, 2024 · Exosomal RNA was extracted with MagZol Reagent (Magen, China). The RNA yield was quantified by using a Qubit fluorometer (Thermo Fisher Scientific, USA) and an Agilent 2200 TapeStation (Agilent Technologies, USA). Briefly, rRNAs were removed from exosomal RNA using a QIAseq FastSelect RNA Removal Kit (QIAGEN, Germany) … WebMay 5, 2024 · To study the spatial and temporal expression, RNA from different tissue parts (head, hemolymph, midgut, epidermis dissected from ten fifth instar larvae) and four to five larvae from different instars was extracted using the Trizol reagent following the MagZol TM Reagent instructions (Magen, Shanghai, China) with three biological replicates ...
Magzol reagent
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WebMar 28, 2024 · Whole brain tissues were harvested and then lysed in Magzol reagent (Magen, China). Groups of 4 mice were used for each virus at one time point. Total RNA of each brain tissue sample was extracted using the HiPure Universal RNA Kit (Magen, China) according to the manufacturer’s protocol. WebMagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction. Details Specifications Principle
WebAug 10, 2024 · Total RNA was isolated from tissues using the Magzol Reagent (Magen, China)according to the manufacturer's protocol. Briefly, total RNAs were ligated with 3' RNA adapter, and followed by 5' adapter ligation. Subsequently, the adapter-ligated RNAs were subjected to RT-PCR and amplified with a low-cycle. Then the PCR products were size … WebMagZolTM Reagent (Trizol Reagent) 简介 MagZolTMReagent为单一相溶液，含异硫氰酸胍和酚。本产品 是在酸性酚异硫氰酸胍RNA提取方案的基础上改良而成的 …
WebBased on this strategy, recombinase polymerase amplification (RPA) and CRISPR-Cas12a detection systems can be integrated into a completely closed test tube. crRNA can be designed to be temporarily inactivated so that RPA is not affected by Cas12a cleavage. WebFeb 9, 2024 · Typically, 10~60 mg tissue was lyzed by grinding with 1 mL MagZol . ... Typically, 10~60 mg tissue was lyzed by grinding with 1 mL MagZol Reagent, followed by the addition of 200.
WebTatol RNA was extracted by Magzol Reagent (Magen, China) Briefly, RNAs were ligated with 3' RNA adapter, and followed by 5' adapter ligation. Subsequently, the adapter-ligated RNAs were subjected to RT-PCR and amplified with a low-cycle. Then the PCR products were size selected by PAGE gel according to instructions of NEBNext® Ultra™ RNA ...
goldcityquartetcastmybreaduponthewaterWebMagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit … hcbs waiver terminationWeb, You can get more details about from mobile site on m.alibaba.com hcbs waiver waiting list iowaWebJun 1, 2024 · RNA was extracted from 12 samples from two melanoma cell lines using MagZol reagent with standard protocol. Overall design: RNA was extracted from 12 samples from two melanoma cell lines using MagZol reagent with standard protocol. We conducted drug sensitivity assays on selected drugs in two different melanoma cell lines … hcbs web toolWebMagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine … gold city quartet cdWebMagZol Reagent (Magen, Guangdong, China) was used to extract total RNA. The miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) (Vazyme, China) was used for reverse transcription PCR for miRNA. hcbs washingtonWebMay 17, 2024 · extracted with Magzol reagent (Invitrogen, Carlsbad, CA, USA). Reverse transcription of mRNA was done using the FastQuant RT Kit (with gDNase; Tiangen Biotech). Quantitative reverse transcription (RT)-PCR analysis was performed with the Fast SYBR Green Master Mix (Invitrogen, Shanghai, China) using synthesized primers from … hcbs waiver wait list iowa