Phospho-akt1/2/3 ser473 cellular kit

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August undefined, 2024

Web寻找 Abcam 抗体及试剂 - Abcam中文官网 WebPhospho-AKT1/2/3 (Ser473) control lysate HTRF®. Phospho-AKT1/2/3 (Ser473) control lysate. This lysate is a component of the phospho-AKT1/2/3 (Ser473) kit. It may be used …

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kits, PerkinElmer

WebPhospho-AKT1 (Ser473) Antibody (700392) in ICC/IF Immunofluorescent analysis of AKT (pS473) was done on 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. WebPhospho-Akt (Ser473) Rabbit mAb has been coated on the microwells. After incubation with cell lysates, phospho-Akt (Ser473) protein is captured by the coated antibody. Following extensive washing, Akt1 Mouse Antibody is added to detect the captured phospho-Akt1 (Ser473) protein. fnaf 3 xbox cheats

Phospho-Akt (Ser473) Antibody Cell Signaling Technology

WebUp-regulation of O-GlcNAcylation increases Akt1 phosphorylation at Ser473. The 8305C cells were treated with 5 μM Thia-G or were transfected with 500 ng OGT plasmid, and then cell lysates were resolved by SDS-PAGE, and phospho-Akt1 (Ser473) or Akt1 were determined with specific antibodies (Figure 4A and B). An increase in Ser473 ... WebPhospho-Akt (Ser473) Antibody detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when … WebThe Phospho-AKT3 (Ser473) kit is designed for the robust quantification of AKT3 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), AKT is an oncogene playing a key role in controlling apoptosis, cell proliferation, transcription, cell migration, and glucose metabolism. greenspire school traverse city mi

Phospho-AKT3 (Ser473) cellular HTRF kit Cisbio - PerkinElmer

PathScan® Phospho-Akt1 (Ser473) Sandwich ELISA Kit

WebThe phospho-EGFR assay kit is designed for the cell-based quantitative detection of Tyr1068 phosphorylation on EGFR. Mutations that lead to an overexpression or hyperactivity of EGFR, also known as ErbB1 or HER1, are associated with a number of cancers, such as lung, colorectal, prostate, glioblastoma cancer, etc. WebPhospho-AKT1/2/3 (Ser473) cellular kit HTRF® Kit Diabetes and metabolic disorders A portfolio to tackle in-depth diabetes and metabolic disorders studies Explore Phospho-AKT1/2/3 (Ser473) control lysate HTRF® Control lysate Phospho-AKT1/2/3 (Thr308) cellular kit HTRF® Kit Phospho-AKT1/2/3 (Thr308) control lysate HTRF® Control lysate Guides greenspire linden tree picturesWebDec 16, 2009 · PIP3 recruits protein dependent kinase 1 (PDK1) and AKT, also known as protein kinase B (PKB), to the plasma membrane where AKT is phosphorylated at Thr308 by PDK1 and at Ser473 by mTOR complex 2 (TORC2) . AKT1 can also be phosphorylated at Ser473 by DNA-PK in the nucleus . AKT occupies a key regulatory node in the PI3K … greenspirit italy

"WebThe Advanced Phospho-ERK assay kits are designed for the robust and highly sensitive quantification of ERK phosphorylation on Thr202/Tyr204 as an MAPK pathway readout. The simple mix-and-read protocol eliminates all wash steps for faster analysis and high quality output. The Total-ERK1/2 assay is compatible with the buffers from the Phospho or ... " - Phospho-akt1/2/3 ser473 cellular kit

Phospho-akt1/2/3 ser473 cellular kit

Phosphorylation - GPCR platform - By universe - Life sciences

WebNov 25, 2015 · The ER also plays a critical role toward maintenance of cellular calcium homeostasis. ... and the following oligonucleotide primers were used for RT-PCR: Akt1 forward, 5′-CTGTCTCGAGAGCGTGTGTT-3′; Akt1 reverse, 5′-AACAGCTTCTCGTGGTCCTG-3′; Akt2 ... [rabbit polyclonal anti-phospho-Akt (Ser473) antibody (Cell Signaling Technology, … WebThe Phospho-AKT3 (Ser473) kit is designed for the robust quantification of AKT3 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), AKT is an …

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WebThis HTRF kit is designed to monitor AKT1 phosphorylation on Ser473 as a readout of Pi3K pathway ... Learn More Phospho-AKT1/2/3 (Ser473) cellular kit

WebApr 14, 2024 · Notably, when proline in the 14-3-3 binding motif was replaced with an alanine (P436A), the 14-3-3 recognition of SIK1 was profoundly attenuated (Supplementary Fig. S4G). Together, these data suggest that AKT-mediated SIK1 phosphorylation enhances SIK1 binding with 14-3-3, which in turn increases the rate of SIK1 transfer to the cytoplasm. WebFeb 3, 2024 · Protein kinase B (AKT1) is hyper-activated in diverse human tumors. AKT1 is activated by phosphorylation at two key regulatory sites, Thr308 and Ser473. Active AKT1 phosphorylates many, perhaps hundreds, of downstream cellular targets in the cytosol and nucleus. AKT1 is well-known for phosphorylatin …

WebThe Total SHP1 cellular assay monitors the expression level of SHP1, and can be used as a normalization assay with our phospho-SHP1 Y564 kit. Because these phospho and total SHP1 assays are compatible, the two kits can be used in parallel on the same lysates. Many cancer cells overexpress checkpoint inhibitor ligands such as PD-L1. WebPhospho-Akt (Ser473) Kit For use with human, mouse, rat, and non-human primate cell lysates and tissue lysates. This package insert must be read in its entirety before using …

WebAKT, also known as protein kinase B, is an important regulator of numerous cellular processes including cell survival, glucose metabolism, transcription, and apoptosis. AKT …

WebThe FastScan™ Phospho-Akt (Ser473) ELISA Kit detects endogenous levels of Akt when phosphorylated at Ser473 as shown in Figure 1. This kit detects proteins from the … greenspire windsor co hoaWebThe Alpha SureFire® Ultra™ HV Multiplex p-AKT 1/2/3 615 (Eu) assay kit is used to measure the phosphorylation of AKT1/2/3 (Ser473) plus another target of choice from the AlphaLISA SureFire ... Akt (1/2/3) at the phospho-Ser473 epitope, and one at a … green spirit clinic addressLANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells. greenspire subdivision windsor coWebPhospho-Akt (Ser473) Antibody detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues. It does not recognize Akt phosphorylated at other sites, nor does it recognize phosphorylated forms of related kinases such as PKC or … fnaf 3 year setWebAKT is a serine/threonine protein kinase that plays a key role in multiple cellular processes including metabolism, proliferation, apoptosis/survival, and migration. There are three homologous isoforms of AKT: AKT1, AKT2, and AKT3. AKT is activated by binding of its pleckstrin homology (PH) domain to membrane phospholipids and by phosphorylation. fnaf 3 year it takes placeWebApr 12, 2024 · Sex related differences have been observed in the progression of Alzheimer’s disease (AD) [1] and the prevalence of AD is greater in women than men in European and American patients [2, 3 ... green spirit made from wormwood crosswordWebIn the case of AKT1/2, immunofluorescence is reduced following the augmentation of NP-427 concentrations (Figure 8A–D). Similar to the anterior cases, the Western blot showed a decrease in phospho-Thr308-Akt, phospho-Ser473-Akt, and total AKT at 24 h in cells treated with NP-427 at 0.5 and 0.75 mg/mL ( p < 0.05) ( Figure 8 E). fnaf 3 year date